Abstract
This application note provides guidelines for choosing CT acquisition settings in a variety of CT imaging
applications for imaging in mice. These guidelines should be treated as a starting point and adjusted
accordingly for specific applications.
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Introduction
CT acquisition on the InSyTe FLECT/CT system includes the animal imaging cell in the field of view. When
the acquired CT data is reconstructed, the animal imaging cell also appears in the CT reconstruction. This
can be problematic when looking at the CT reconstruction as visualizing the imaging cell is not necessary.
This application note outlines the steps to remove the imaging cell from the reconstructed CT image using
VivoQuant.
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The InSyTe FLECT/CT system acquires separate X-ray CT and FLECT images. Fusion of the FLECT and CT
images gives combined molecular and anatomical information in a single image. Due to a mismatch in the
geometric center of each subsystem, when fused together, the FLECT and CT images are misaligned. This
note outlines the steps to first fuse together and then manually co-register the X-ray CT and FLECT images
using VivoQuant.
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Abstract Optical imaging is an established approach for studying cancer in preclinical animal models. Near infrared fluorescence tomography is an emerging optical imaging modality that enables 3D visualization and deep tissue imaging capabilities. Commercially available fluorescent imaging probes further enable imaging of specific physiology that informs disease state and progression. ….
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