True 360° Tomographic Optical Preclinical Imaging
With the FLECT system, TriFoil enables researchers to realize the full potential of in vivo optical imaging for small animals. FLECT allows researchers to capture true 3D tomographic images by acquiring projection images 360° around the animal subject and sequent accurate image reconstruction. With the FLECT system, researchers will no longer have to choose
between imaging only near the surface of the animal subject and partial tomography solutions that distort the subject or create artifacts. Instead, the FLECT provides uniform sensitivity and resolution throughout the animal subject. For the first time, researchers will be able to examine deep tissue phenomena such as spontaneous metastases without sacrificing image quality or quantitative accuracy.
With the optional upgrade of an integrated, inline X-ray CT, researchers will gain anatomical correlation data that will significantly improve the segmentation and resolution of the optical images. Our InSyTe FLECT/CT™ system automatically co-registers the optical and CT images for better anatomical correlation.
FLECT (Fluorescence Emission Computed Tomography) reconstructs the 3D tomographic images from the photon emission data acquired 360° around the animal subject (projection images) using a state-of-the-art 3D reconstruction engine based on an accurate model of light propagation in biological tissue. The result is the most accurate optical quantitation available.
The FLECT laser excites the fluorophore inside the animal subject, causing the fluorophore to emit light at a different wavelength, or fluorescence. The fluorescence is recorded by an array of detectors around the animal subject. Then the gantry rotates, changing the position of the laser and detectors, for acquiring data at a different angle. This process is repeated again and again until the subject has been imaged at all angles covering 360° for each individual slice. The animal bed is then moved to the next slice position so that the animal subject can be imaged along its entire body.
Features & Benefits
- Uniform superior sensitivity and high resolution high resolution throughout the animal subject
- Fewer assumptions/artifacts in 3D image reconstruction
- Accurate measures of fluorophore concentrations throughout the entire animal
- Examination of deep tissue phenomena such as spontaneous metastases without sacrificing image quality or quantitative accuracy
- No distortion of body shape - true anatomical displacement of animal
- Laser and filter customization allows flexibility and smooth workflow
- Core application areas include Oncology, Cardiovascular, Inflammation, Pulmonology and Skeletal Disease